E Coli Liquid Culture. Coli that reach stationary phase with overnight incubation. 2) check that culture looks pure.
If so, you will likely need to grow for a longer time to get the correct density of bacteria since they will grow more. Coli proteins within microenvironments surrounding single. Jm109, top10, dh5 ) from glycerol stock or other source onto plain lb agar (no antibiotics).